Journal
JOURNAL OF PROTEOME RESEARCH
Volume 8, Issue 4, Pages 1953-1964Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr800897c
Keywords
Arthrobacter chlorophenolicus; 4-chlorophenol; 4-nitrophenol; phenol; shotgun proteomics; 2D-LC-ES-MS/MS; hydroxyquinol dioxygenase
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Funding
- Swedish University of Agricultural Sciences, Uppsala, Sweden
- UT-Battelle, LLC [DE-AC05-00OR22725]
- Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
- US Department of Energy [DE-AC02-05-CH11231]
- DOE Joint Genome Institute, Walnut Creek, CA
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We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
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