Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 9, Pages 3789-3802Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr800233r
Keywords
oxidation; DIGE; ICAT; cysteine thiol; redox proteomics
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Funding
- NIH [NS046593]
- foundation of UMDNJ
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Oxidative modifications of protein thiols are important mechanisms for regulating protein functions. The present study aimed to compare the relative effectiveness of two thiol-specific quantitative proteomic techniques, difference gel electrophoresis (DIGE) and isotope coded affinity tag (ICAT), for the discovery of redox-sensitive proteins in heart tissues. We found that these two methods were largely complementary; each could be used to reveal a set of unique redox-sensitive proteins. Some of these proteins are low-abundant signaling proteins and membrane proteins. From DIGE analysis, we found that both NF-kappa B-repressing protein and epoxide hydrolase were sensitive to H2O2 oxidation. In ICAT analysis, we found that specific cysteines within sacroplasmic endoplamic reticulum calcium ATPase 2 and voltage-dependent anion-selective channel protein 1 were sensitive to H2O2 oxidation. From these analyses, we conclude that both methods should be employed for proteome-wide studies, to maximize the possibility of identifying proteins containing redox-sensitive cysteinyl thiols in complex biological systems.
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