Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 8, Pages 3543-3555Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr800286z
Keywords
ethanol preserved; tissue; proteins; imaging mass spectrometry; MALDI
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Funding
- NCI NIH HHS [R33 CA116123, P30 CA068485-109002, 5 P30 CA 068485-10, P30 CA068485, R33 CA116123-03, 5 R33 CA 116123-3] Funding Source: Medline
- NIGMS NIH HHS [R01 GM058008, 5 R01 GM 058008-09, R01 GM058008-09] Funding Source: Medline
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Imaging mass spectrometry is becoming a key technology for the investigation of the molecular content of biological tissue sections in direct correlation with the underlying histology. Much of our work has been done with fresh-frozen tissue sections that has undergone minimal protein degradation between the time a tissue biopsy is sampled and the time it is snap-frozen so that no preserving or fixing agents need to be added to the frozen biopsy. However, in many sampling environments, immediate flash freezing may not be possible and so we have explored the use of ethanol-preserved, paraffin-embedded tissue specimens for proteomic analyses. Solvent-only preserved tissue specimens provide long-term preservation at room temperature, generation of high quality histological sections and little if any chemical alteration of the proteins. Using mouse organs, several key steps involved in the tissue dehydration process have been investigated to assess the potential of such preserved specimens for profiling and imaging mass spectrometry investigations.
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