Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 7, Pages 3060-3063Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr8001029
Keywords
human erythrocyte proteome; hemoglobin depletion; carbonic anhydrase-1 depletion; erythrocyte disorder proteomics; IEX-chromatography; Ni(II) chromatography; multi-dimensional liquid chromatography
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In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood.
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