Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 8, Pages 3423-3427Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr8001595
Keywords
-
Categories
Funding
- Intramural NIH HHS [Z99 DA999999] Funding Source: Medline
Ask authors/readers for more resources
We have previously used MALDI mass spectrometry to highlight ammonium- or guaniclinium-aromatic interactions via cation-pi bonding and ammonium- or guanidinium-phosphate interactions through salt bridge formation. In the present work, the gas-phase stability and dissociation pathways of the interaction between phosphorylated peptides and compounds containing quaternary amines are demonstrated using electrospray ionization mass spectrometry. The presence of one quaternary amine in a compound is enough to form a noncovalent complex with a phosphorylated residue. However, if two quaternary amines are present in one molecule, the electrostatic interactions of the quaternary amines with the phosphate results in a covalent-like stability, and these bonds can withstand fragmentation by collision-induced dissociation at energies similar to those that fragment covalent bonds. Such interactions are important in accounting for physiological, pathophysiological, and pharmacological effects of many therapeutic compounds and small molecules containing quaternary amines or phosphates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available