Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 6, Pages 2562-2568Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr800066e
Keywords
protein O-glycosylations; plasma; epithelial ovarian cancer (EOC); matrix-assisted laser desorption/iozation (MALDI); Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR-MS)
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Funding
- NATIONAL CANCER INSTITUTE [R33CA105295] Funding Source: NIH RePORTER
- NCI NIH HHS [R33 CA105295] Funding Source: Medline
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Recent investigations have implicated aberrant glycosylations in various malignancies, including epithelial ovarian cancer (EOC). The protocol here identifies O-linked carbohydrate patterns in EOC plasma glycoproteins through chemical cleavage and purification of these glycans. Dialyzed plasma is subjected to reductive beta-elimination with alkaline borohydride to release O-linked oligosaccharides from glycoproteins. Enrichment of released glycans, as well as removal of peptide and other contaminants, is followed by carbohydrate pattern analysis with MALDI-FT-ICR-MS.
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