Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 11, Pages 4869-4877Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr800473j
Keywords
Phosphopeptide enrichment; EGFR signaling; A431 carcinoma cells; WAX; IMAC; ERLIC; SCX
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Funding
- National Cancer Centre Singapore Research Foundation
- Biomedical Research Council (BMRC) [07/1/22/19/531]
- Ministry of Education of Singapore [T206B3211]
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Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.
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