Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 6, Pages 2546-2552Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr7007219
Keywords
protein fractionation; visualization LC-MS/MS; isoforms
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We developed a visualization approach for the identification of protein isoforms, precursor/mature protein combinations, and fragments from LC-MS/MS analysis of multidimensional fractionation of serum and plasma proteins. We also describe a pattern recognition algorithm to automatically detect and flag potentially heterogeneous species of proteins in proteomic experiments that involve extensive fractionation and result in a large number of identified serum or plasma proteins in an experiment. Examples are given of proteins with known isoforms that validate our approach and present a subset of precursor/ mature protein pairs that were detected with this approach. Potential applications include identification of differentially expressed isoforms in disease states.
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