Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 3, Pages 1244-1250Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr070451j
Keywords
Escherichia coli; bacteriophage; RNA polymerase; bacteriophage infection; affinity isolation; MALDI MS
Categories
Funding
- NCRR NIH HHS [P41 RR000862-35, U54 RR022220-046885, U54 RR022220, RR00862, P41 RR000862] Funding Source: Medline
- NIGMS NIH HHS [R01 GM061898, GM61898, R01 GM061898-08, GM59295, R01 GM062427, R01 GM059295-08, R01 GM059295, R01 GM062427-08] Funding Source: Medline
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Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.
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