Journal
JOURNAL OF PROTEOME RESEARCH
Volume 7, Issue 7, Pages 2703-2711Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr700780x
Keywords
tandem mass spectrometry; vaccinia virus; epitope; antigen processing; antigen presentation; peptide binding; T cell response; CD4(+) T-cell; immune response; vaccine
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Funding
- NIAID NIH HHS [U19 AI057319-019001, U19 AI057319-010004, U19 AI057319, R21 AI074616-02, R21 AI074616, R21 AI074616-01A1] Funding Source: Medline
- NIDDK NIH HHS [DK32520, P30 DK032520] Funding Source: Medline
- PHS HHS [NIH-U19-057319] Funding Source: Medline
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Class II MHC proteins bind peptides and present them to CD4(+) T cells as part of the immune system's surveillance of bodily tissues for foreign and pathogenic material. Antigen processing and presentation pathways have been characterized in detail in normal cells, but there is little known about the actual viral peptides that are presented to CD4(+) T cells that signal infection. In this study, two-dimensional LC-MS/MS was used to identify vaccinia virus-derived peptides among the hundreds to thousands of peptide antigens bound to the human class 11 MHC protein HLA-DR1 on the surface of vaccinia virus-infected cells. The peptides, derived from the 16L, D6R, and A10L viral proteins, were 15 residues in length, bound efficiently to HLA-DR1 as synthetic peptides, and were recognized by vaccinia-specific CD4(+) T cells obtained from an immunized donor.
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