4.5 Article

Molecular cloning and characterization of disease-resistance gene candidates of the nucleotide binding site (NBS) type from Cocos nucifera L

Journal

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
Volume 89, Issue -, Pages 87-96

Publisher

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pmpp.2015.01.002

Keywords

Coconut palm; Lethal yellowing; R genes

Categories

Funding

  1. CONACyT, Mexico [CB 129717]
  2. CONACyT [47245]
  3. Common fund for Commodities, Stadhouderskade 55, 1072 AB Amsterdam [FIG00/22]

Ask authors/readers for more resources

In this study, degenerate primers were used to amplify nucleotide-binding site ( NBS)-type sequences from coconut ecotypes either resistant or susceptible to lethal yellowing. Genomic DNA fragments of approximately 500-700 bp were obtained and sequenced. Phylogenetic analysis of these fragments revealed that they clustered in seven different clades. We also found that all CnRGC sequences were grouped within the non-TIR-NBS-LRR subclass of NBS-LRR genes. The expression analysis revealed changes in expression profiles in response to salicylic acid ( SA) and a constitutive expression profile in plants untreated with SA. This is the first large scale analysis of NBS-type sequences in coconut palm. (C) 2015 Elsevier Ltd. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available