Journal
JOURNAL OF POROUS MATERIALS
Volume 21, Issue 1, Pages 99-104Publisher
SPRINGER
DOI: 10.1007/s10934-013-9752-9
Keywords
Zinc (II) nitrate; BDC; Pendent group; Bioconjugation; Photoluminescence quenching; Bovine serum albumin
Funding
- CSIR, India [OMEGA/PSC0202/2.2.5]
- CSIR
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Zn-BDC (BDC = 1,4-benzenedicarboxylate acid) or more commonly known as metal organic framework (MOF-5) was synthesized via chemical stirring method and bioconjugated with anti-bovine serum albumin (anti-BSA) through pendent group. The pendent group of organic linker on surface of MOF-5 has been activated via EDC (1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide) based simple organic reaction and then bioconjugation of anti-BSA was achieved. Bioconjugation of anti-BSA on to MOF-5 has been used to study the interaction with BSA based on their fluorescence properties. Fluorescent microscopy revealed successful interaction of anti-BSA with BSA onto MOF-5 surface. In addition, BSA conjugated MOF-5 showed no loss of enantioselectivity and structure stability. Photoluminescence quenching of the developed probe was demonstrated to observe the presence of BSA.
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