4.5 Article

Fabrication of hydrophilic nanoporous PMMA/O-MMT composite microfibrous membrane and its use in enzyme immobilization

Journal

JOURNAL OF POROUS MATERIALS
Volume 20, Issue 3, Pages 457-464

Publisher

SPRINGER
DOI: 10.1007/s10934-012-9615-9

Keywords

Electrospinning; Nanoporous; Cold plasma; Laccase; Adsorption

Funding

  1. National High-tech R&D Program of China [2012AA030313]
  2. Changjiang Scholars and Innovative Research Team in University [IRT1135]
  3. National Natural Science Foundation of China [51006046, 51163014]
  4. Priority Academic Program Development of Jiangsu Higher Education Institutions
  5. Specialized Research Fund for the Innovation Project of Jiangsu Graduate Education [CXZZ11_0471]
  6. Research Fund for Doctoral Program of Higher Education of China [20090093110004]

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The electrospun PMMA/O-MMT microfibrous membranes were pretreated by oxygen plasma to create substrates with better adsorption capability. The amount of O-MMT and conditions of plasma treatment were optimized for maximum laccase immobilization on these pretreated surfaces of the microfibrous membranes. The surface morphology and chemistry of the composite microfibrous membranes after plasma treatment and laccase immobilization were investigated by SEM and FTIR. The immobilized laccase showed better resistance to pH and temperature changes than that of the free form laccase, and after 10 successive runs of repeated use, the immobilized laccase still retained 30 % of its initial activity. Reactive X-3B was successfully degraded by both free and immobilized laccase.

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