Synthesis and Properties of Chitosan-Based Thermo- and pH-Responsive Nanoparticles and Application in Drug Release

In this research, thermo- and pH-responsive nanoparticles with an average diameter of about 50-200 nm were synthesized via the surfactant-free emulsion polymerization. The thermal/pH dual responsive properties of these nanoparticles were designed by the addition of a pH sensitive monomer, acrylic acid (AA), to be copolymerized with N-isopropylacrylamide (NIPAAm) in a chitosan (CS) solution. The molar ratio of CS/AA/NIPAAm in the feed was changed to investigate its effect on structure, morphology, thermal- and pH-responsive properties of the nanoparticles. It was found that CS-PAA-PNIPAAm nanoparticles could be well dispersed in the aqueous solution and carried positive charges on the surface. The addition of thermal-sensitive NIPAAm monomer affected the polymerization mechanism and interactions between CS and AA. The particle size of the nanoparticles was found to be varied with the composition of NIPAAm monomer in the feed. The synthesized nanoparticles exhibited stimuli-responsive properties, and their mean diameter thus could be manipulated by changing pH value and temperature of the environment. The nanoparticles showed a continuous release of the encapsulated doxycycline hyclate up to 10 days during an in vitro release experiment. The environmentally responsive nanoparticles are expected to be used in many fields such as drug delivery system. (c) 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 2798-2810, 2009