Journal
JOURNAL OF PLANT RESEARCH
Volume 126, Issue 3, Pages 439-446Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s10265-012-0537-z
Keywords
Chara; Depolarization; Dimethyl sulfoxide; Ethylene glycol; Membrane potential; Osmolality; Turgor pressure
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By taking advantage of large cell size of Chara corallina, we analyzed the membrane depolarization induced by decreased turgor pressure (Shimmen in J Plant Res 124:639-644, 2011). In the present study, the response to increased turgor pressure was analyzed. When internodes were incubated in media containing 200 mM dimethyl sulfoxide, their intracellular osmolality gradually increased and reached a steady level after about 3 h. Upon removal of dimethyl sulfoxide, turgor pressure quickly increased. In response to the increase in turgor pressure, the internodes generated a transient membrane depolarization at its nodal end. The refractory period was very long and it took about 2 h for full recovery after the depolarizing response. Involvement of protein synthesis in recovery from refractoriness was suggested, based on experiments using inhibitors.
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