Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 168, Issue 14, Pages 1609-1616Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2011.03.007
Keywords
Betaine transport; Choline transporter; Proline transporter; Salt stress; Sugar beet
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Funding
- Ministry of Education, Science and Culture of Japan
- Salt Science Research Foundation
- High-Technology Research Center of Meijo University
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Proline transporters (ProTs) originally described as highly selective transporters for proline, have been shown to also transport glycinebetaine (betaine). Here we examined and compared the transport properties of Bet/ProTs from betaine accumulating (sugar beet,Amaranthus, and Atriplex,) and non-accumulating (Arabidopsis) plants. Using a yeast mutant deficient for uptake of proline and betaine, it was shown that all these transporters exhibited higher affinity for betaine than proline. The uptake of betaine and proline was pH-dependent and inhibited by the proton uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP). We also investigated choline transport by using a choline transport-deficient yeast mutant. Results revealed that these transporters exhibited a higher affinity for choline uptake rather than betaine. Uptake of choline by sugar beet BvBet/ProT1 was independent of the proton gradient and the inhibition by CCCP was reduced compared with that for uptake of betaine, suggesting different proton binding properties between the transport of choline and betaine. Additionally, in situ hybridization experiments revealed the localization of sugar beet ByBet/ProT1 in phloem and xylem parenchyma cells. (C) 2011 Elsevier GmbH. All rights reserved.
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