Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 167, Issue 5, Pages 333-342Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2009.10.013
Keywords
Coffea sp.; Cold; Oxidative stress; Reactive oxygen species; Scavenging molecules
Categories
Funding
- FCT [REEQ/374/B10/2005, POCTI/AGG/43101/2001, PTDC/AGR-AAM/64078/2006]
- European Fund FEDER
- Portuguese PIDDAC [SFRH/BPD/21884/2005, SFRH/18361/2004]
- European Social Fund
Ask authors/readers for more resources
Low positive temperature (chilling) is frequently linked to the promotion of oxidative stress conditions, and is of particular importance in the coffee plant due to its severe impact on growth, development, photosynthesis and production. Nevertheless, some acclimation ability has been reported within the Coffea genus, and is possibly related to oxidative stress control. Using an integrated biochemical and molecular approach, the characterization of the antioxidative system of genotypes with different cold acclimation abilities was performed. Experiments were carried out using 1.5-year-old coffee seedlings of Coffea canephora cv. Apoata, C arabica cv. Catuai, C dewevrei and 2 hybrids, Icatu (C arabica x C canephora) and Plata (C dewevrei x C arabica) subjected to a gradual cold treatment and a recovery period. Icatu showed the greatest ability to control oxidative stress, as reflected by the enhancement of several antioxidative components (Cu,Zn-SOD and APX activities; ascorbate, a-tocopherol and chlorogenic acids (CGAs) contents) and lower reactive oxygen species contents (H2O2 and OH center dot). Gene expression studies show that GRed, DHAR and class III and IV chitinases might also be involved in the cold acclimation ability of Icatu. Catuai showed intermediate acclimation ability through the reinforcement of some antioxidative molecules, usually to a lesser extent than that observed in Icatu. On the other hand, C. dewevrei showed the poorest response in terms of antioxidant accumulation, and also showed the greatest increase in OH center dot values. The difference in the triggering of antioxidative traits supports the hypothesis of its importance to cold (and photoinhibition) tolerance in Coffea sp. and could provide a useful probe to identify tolerant genotypes. (C) 2009 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available