Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 167, Issue 5, Pages 358-364Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2009.09.011
Keywords
Endo-pectate lyase; Homogalacturonan; Pectin structure; Protonema; Scopelophila cataractae
Categories
Funding
- Japan Society for the Promotion of Science [20580363]
- Yakumo Foundation for Environmental Science
- Oohara Foundation for Agricultural Sciences
Ask authors/readers for more resources
The growth kinetics in the presence of copper (Cu) of the protonema of the moss Scopelophila cataractae and the matrix polysaccharides of its cell walls have been analyzed in this study. Protonemal cells cultured in a medium containing 0.2 mM CuSO4 showed a rapid accumulation of Cu, reaching a maximum between 30 and 60 d at approximately 65 mu mol g(-1) DW. Uronic acids were found in similar amounts in cell walls of both control and Cu-treated cells, whereas arabinose and galactose decreased to 61-67% in the presence of Cu. Cell wall polysaccharides were determined after successive extraction with 50 mM CDTA, 50 mM Na2CO3, 1 M KOH, and 4 M KOH. The pectic fractions (CDTA- and Na2CO3-soluble) decreased to 47% and the hemicellulosic fractions (1 M KOH- and 4 M KOH-soluble) to 86% under Cu application. Approximately 43% of the Cu taken into cell walls was released following endo-pectate lyase treatment, suggesting that two-fifths of the total Cu accumulation was tightly bound to the homogalacturonan of the cell wall pectin. (C) 2009 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available