Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 166, Issue 6, Pages 588-601Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2008.09.003
Keywords
Bentgrass; Candidate genes; Grass; High temperature
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Funding
- United States Golf Association and Rutgers Center of Turfgrass Science
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To gain insights into molecular mechanisms of grass tolerance to heat stress, we constructed a suppression subtractive cDNA library to identify heat-responsive genes for a C-3 grass species, thermal Agrostis scabra adapted to heat stress in geothermal areas in Yellowstone National Park. Plants were exposed to 20 degrees C (control) or 35 degrees C for 12d. The SSH analysis was performed with control samples as the driver and heat-stressed samples as the tester. Differentially expressed cDNA fragments were cloned to screen the heat up-regutated library. The SSH analysis identified 120 non-redundant putative heat-responsive cDNAs; out of 1180 clones. Genes with homology to known proteins were categorized into six functional groups, with the largest group of genes involved in stress/defense, followed by the group of genes related to protein metabolism. Immunoblot analysis confirmed increases in transcripts of selected genes under heat stress. Transcripts of seven and eight genes were strongly enhanced or induced in shoots and roots, respectively, white two genes were only induced in roots under heat stress. The heat up-regulated genes in thermal A. scabra adapted to tong-term heat stress are potential candidate genes for engineering stress-tolerant grasses and for revealing molecular mechanisms of grass adaptation to heat stress. (C) 2008 Elsevier GmbH. All rights reserved.
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