Journal
JOURNAL OF PLANT PHYSIOLOGY
Volume 165, Issue 11, Pages 1168-1180Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2007.09.006
Keywords
cell wall polysaccharides; drought stress; root; Triticum durum; wheat
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Glycosyl composition and linkage analysis of cell watt polysaccharides were examined in apical root zones excised from water-stressed and unstressed wheat seedlings (Triticum durum Desf.) cv. Capeiti (drought-tolerant) and cv. Creso (drought sensitive). Wall polysaccharides were sequentially solubilized to obtain three fractions: CDTA+Na2CO3 extract, KOH extract and the insoluble residue (alpha-cellulose). A comparison between the two genotypes showed only small variations in the percentages of matrix polysaccharides (CDTA+Na2CO3 plus KOH extract) and of the insoluble residues (alpha-cellulose) in water-stressed and unstressed conditions. Xylosyl, glucosyl and arabinosyl residues represented more than 90 mol% of the matrix polysaccharides. The linkage analysis of matrix polysaccharides showed high levels of xyloglucans (23-39 mol%), and arabinoxylans (38-48 mol%) and a low amount of pectins and (1 -> 3), (1 -> 4)-beta-D-glucans. The high level of xyloglucans was supported by the release of the diagnostic disaccharide isoprimeverose after Driselase digestion of KOH-extracted polysaccharides. In the drought-toterant cv. Capeiti the mol% of side chains of rhamnogalacturonan I and II significantly increased in response to water stress, whereas in cv. Creso, this increase did not occur. The results support a role of the pectic side chains during water stress response in a drought-tolerant wheat cultivar. (c) 2007 Elsevier GmbH. All rights reserved.
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