Classification of rice genotypes based on their mechanisms of adaptation to iron toxicity

Iron (Fe) toxicity is a nutritional disorder that affects lowland rice (Oryza sativa L.). The occurrence of excessive amounts of reduced Fe(II) in the soil solution, its uptake by the rice roots, and its transpiration-driven transport result in elevated Fe(II) concentrations in leaf cells that catalyze the formation of reactive oxygen species. The oxidative stress causes rusty brown spots on leaves (bronzing) and the reduction of biomass and yield. While the use of resistant genotypes is the most promising approach to address the problem, the stress appears to differentially affect rice plants as a function of plant age, climatic conditions, stress intensity and duration, and the prevailing adaptation mechanism. We comparatively assessed 21 contrasting 6-week-old rice genotypes regarding their response (symptom score, biomass, Fe concentrations and uptake) to a 6 d iron pulse of 1500 mg L1 Fe(II). Eight selected genotypes were further compared at different stress intensities (0, 500, 1000, and 1500 mg L1 Fe(II)) and at different developmental stages (4-, 6-, and 8-week-old plants). Based on Fe-induced biomass reduction and leaf-bronzing score, the tested spectrum was grouped in resistant and sensitive genotypes. Linking bronzing scores to leaf iron concentrations allowed further differentiation into includer and excluder types. Iron precipitation on roots and organ-specific iron partitioning permitted to classify the adaptation strategies into root exclusion, stem and leaf sheath retention, and leaf blade tissue tolerance. The effectiveness of these strategies differed with stress intensity and developmental stage. The reported findings improve the understanding of Fe-stress response and provide a basis for future genotype selection or breeding for enhancing Fe-toxicity resistance in rice.