Journal
JOURNAL OF PLANT GROWTH REGULATION
Volume 33, Issue 2, Pages 355-363Publisher
SPRINGER
DOI: 10.1007/s00344-013-9386-9
Keywords
Primary root growth; Meristem zone; Elongation zone; Stem cell; Auxin biosynthesis
Categories
Funding
- National Natural Science Foundation [31071339, 31000121]
- National Basic Research Program in China [2012CB114200]
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Wali7 domain-containing protein has been isolated from roots and root hairs. However, the function of this protein has not been reported yet. This study demonstrated that overexpression of a gene encoding Arabidopsis Wali7 domain-containing protein, called auxin-mediated short root (ASR), produced a short-root phenotype. Expression analysis indicated that ASR was induced by auxin. ASR overexpression resulted in a lower level of auxin in transgenic plant roots as indicated by lower DR5::b-glucuronidase (GUS) activity and lower expressions of genes encoding the rate-limited enzymes of auxin synthesis. Our results also suggested that ASR overexpression caused decreased meristematic activity and impaired stem cell division as indicated by CycB1:1::GUS activity and lower expression of a plethora of stem cell transcription factors, respectively. In addition, exogenous application of auxin (NAA and indole-3-acetic acid) could repair root development defects. ASR overexpression decreased auxin levels in transgenic Arabidopsis roots, caused decreased meristematic activity and impaired stem cell division, and resulted in a short-root phenotype.
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