Journal
JOURNAL OF PLANT BIOLOGY
Volume 56, Issue 2, Pages 85-90Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s12374-013-0905-9
Keywords
Activation tagging; Insertion mutagenesis; Handling methods; Rice; T-DNA
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Funding
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ008215]
- Basic Research Promotion Fund, Republic of Korea [2012-055219]
- Kyung Hee University [20120227]
- National Research Foundation of Korea [2007-0093862] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [PJ008215012013] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We have previously developed more than 100,000 T-DNA insertion mutant populations in japonica rice. These include simple knockouts as well as those for activation tagging. T-DNA insertion sites have been determined from more than 50,000 lines. The database for insertion positions is now open to the public, and these tagging lines are widely distributed to members of the rice research community. To utilize these genetic resources more efficiently, we are summarizing the important features of these tagging vectors, rice varieties, and flanking sequences. We also provide methods for handling such materials.
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