Journal
JOURNAL OF PLANT BIOLOGY
Volume 54, Issue 5, Pages 294-302Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s12374-011-9166-7
Keywords
Oryza species; Rice blast; Pi9; Polymorphism; Evolution; Rice resistance gene; Magnaporthe oryzae
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Funding
- 973 Project [2006CB101904]
- 948 Project [2006-G61]
- Ministry of Agriculture
- National Natural Science Foundation of China [30571063]
- World Class University (WCU), Korean Ministry of Education, Science and Technology
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The rice nucleotide-binding site-leucine-rich repeat (NBS-LRR)-encoding resistance (R) gene Pi9 confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae. The Pi9 locus comprises many NBS-LRR-like genes and is an ancient locus that is highly conserved in cultivated and wild rice species. To understand the genetic variation and molecular evolutionary mechanism of the Pi9 alleles in different rice species, we studied five AA genome Oryza species including two cultivated rice species (Oryza sativa and Oryza glaberrima) and three wild rice species (Oryza nivara, Oryza rufipogon, and Oryza barthii). A 2.9-kb fragment spanning the NBS-LRR core region of the Pi9 gene was amplified and sequenced from 40 accessions. Sequence comparison revealed that the Pi9 alleles had an intermediate-diversified nucleotide polymorphism among the AA genome Oryza species. Sequence variations were more abundant in the LRR region than in the NBS region, indicating that the LRR region has played a more important role for the evolution of the Pi9 alleles. Furthermore, positive selection was found to be the main force promoting the divergence of the Pi9 alleles, especially in the LRR region. Our results reveal the characteristics and evolutionary dynamics of the Pi9 alleles among the two cultivated and three wild rice species.
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