4.1 Article

AFLP and RAPD based genetic diversity assessment of industrially important reed bamboo (Ochlandra travancorica Benth)

Journal

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 22, Issue 1, Pages 144-149

Publisher

SPRINGER INDIA
DOI: 10.1007/s13562-012-0114-5

Keywords

AFLP; AMOVA; Genetic diversity; Reed bamboo

Funding

  1. NMITLI Cell of CSIR
  2. Department of Biotechnology, New Delhi, India

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A high level of genetic diversity was recorded in molecular analysis of random collections of industrially important reed bamboos (O. travancorica) from Kerala region of India. Fifty primers (8 AFLP & 42 RAPD) detected 914 polymorphic loci. Cluster and Principal Coordinate Analysis (PCA) based on combined AFLP & RAPD data grouped all the random accessions into three different populations. Further, AMOVA revealed a moderate to high level of genetic variation. Most of the variation occurred within population (54 %) than among population (46 %). Highly significant and high PhiPT estimate (Fst value; 0.456) indicated that these populations are not panmictic and are significantly isolated. Relatively large number of polymorphism obtained could be useful in finding the elite germplasm resources for industrial use.

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