PCR-based characterization of mung bean (Vigna radiata) genotypes from Indian subcontinent at intra- and inter-specific level

Identification and successful deployment of efficient molecular markers can minimize ambiguity in varietal identification and registration in mung bean (Vigna radiata). Eighty accessions of mung bean having diverse origin and nature were classified based on agro-morphological traits. Using fifteen representative accessions, ten PCR-based efficient primers, were identified with higher polymorphic information content (PIC) values and higher Marker Indexes (Mill. Even the combination of only two primers was found sufficient to differentiate all fifteen genotypes. In addition, it was observed that one resistance gene analogue (RGA) primer, designed from soybean, could able to amplify 445bp fragment only in MYMV tolerant lines of mung bean. One mung bean genotype (B1) was differentiated from wild and cultivated accessions belonging to twelve other indigenous Vigna species and subspecies by employing one each of microsatellite, ISSR and RAPID primers. One decamer (5' GTC GCC GTC A 3') could successfully identity an inter-sub-specific hybrid as well. The highly informative primers identified in this study, could be utilized in generating useful molecular descriptors for fingerprinting mung bean genotypes from Indian Subcontinent.