Journal
JOURNAL OF PHYTOPATHOLOGY
Volume 160, Issue 11-12, Pages 755-757Publisher
WILEY
DOI: 10.1111/jph.12007
Keywords
willow proliferation; group 16SrVI phytoplasma; China
Categories
Funding
- 111 project
- Ph.D. Program Foundation from the Ministry of Education of China [B07049, 20100204110004]
- National Natural Science Foundation of China [30970133]
- China Ministry of Education [Z107021202]
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In 2010 and 2011, willow proliferation disease was observed in Erdos, Inner Mongolia, China. The phytoplasma-specific 16S rRNA gene fragment of 1.2 kb was amplified by a nested PCR with universal primer pair P1/P7 followed by R16F2n/R2. Phylogenetic and virtual RFLP analyses revealed that the phytoplasma associated with willow proliferation was a member of subgroup 16SrVI-A. The field survey indicated that the incidence of willow proliferation in Erdos was approximately 36.84%. To our knowledge, this is the first record of group 16SrVI phytoplasma infecting willow in China.
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