Journal
JOURNAL OF PHYTOPATHOLOGY
Volume 160, Issue 11-12, Pages 622-627Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1439-0434.2012.01943.x
Keywords
banana; virus; multiplex PCR; detection; China
Categories
Funding
- General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China [2009IK265]
Ask authors/readers for more resources
Banana bunchy top virus (BBTV), Cucumber mosaic virus (CMV), Banana bract mosaic virus (BBrMV) and Banana streak virus (BSV) are four viruses known to infect banana corp. On the basis of the establishment of the optimal PCR and reverse transcription (RT)-PCR for the detection of a single virus, a quadruplex RT-PCR method that employed virus-specific primers was developed for the simultaneous detection and differentiation of all the four viruses in banana. Several sets of primers for each target virus were evaluated for their sensitivity and specificity by simplex and quadruplex RT-PCR. The assay was then validated using banana samples infected with one or more viruses collected from fields and tissue-culture banana industries in Hainan Island.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available