Journal
JOURNAL OF PHYTOPATHOLOGY
Volume 158, Issue 11-12, Pages 750-757Publisher
WILEY
DOI: 10.1111/j.1439-0434.2010.01695.x
Keywords
microbial plant pathogens; nucleic acids extraction; PCR; RT-PCR; FTA cards
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For the detection of microbial plant pathogens, like fungi, bacteria, viruses and viroids, methods based on nucleic acids have gained importance as the availability of sequence information increased. This requires well-established extraction procedures that are cheap, non-laborious, safe and reliable. The paper cards introduced by Flinders Technology Associates, acronym FTA (R) cards, offer a simple tool to sample and preserve nucleic acids from many kinds of biological specimen and have been already tested for their potential to sample and process several plant pathogens in PCR and RT-PCR. We have tested FTA cards for the sample preparation of a broader range of plant pathogens with different NA contents and subsequent amplification by PCR, RT-PCR as well as multiplex PCR.
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