Journal
JOURNAL OF PHYSIOLOGY-LONDON
Volume 590, Issue 1, Pages 99-107Publisher
WILEY-BLACKWELL
DOI: 10.1113/jphysiol.2011.219014
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Funding
- Swiss National Science Foundation [PZ00P3 126388, 310030-1270911]
- EU [223524, 243914]
- Swiss SystemsX.ch initiative
- Swiss National Science Foundation (SNF) [PZ00P3_126388] Funding Source: Swiss National Science Foundation (SNF)
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In vivo optical imaging can reveal the dynamics of large-scale cortical activity, but methods for chronic recording are limited. Here we present a technique for long-term investigation of cortical map dynamics using wide-field ratiometric fluorescence imaging of the genetically encoded calcium indicator (GECI) Yellow Cameleon 3.60. We find that wide-field GECI signals report sensory-evoked activity in anaesthetized mouse somatosensory cortex with high sensitivity and spatiotemporal precision, and furthermore, can be measured repeatedly in separate imaging sessions over multiple weeks. This method opens new possibilities for the longitudinal study of stability and plasticity of cortical sensory representations.
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