Journal
JOURNAL OF PHYSIOLOGY-LONDON
Volume 588, Issue 20, Pages 3921-3931Publisher
WILEY-BLACKWELL
DOI: 10.1113/jphysiol.2010.192096
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Funding
- MRC
- BBSRC
- Medical Research Council [G0500198] Funding Source: researchfish
- MRC [G0500198] Funding Source: UKRI
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We have previously shown connexin mediated CO2-dependent ATP release from the surface of the medulla oblongata. Given the localization of connexin 26 (Cx26) to the chemosensing areas of the medulla, we have tested in a heterologous expression system (HeLa cells) whether Cx26 may be sensitive to changes in P-CO2. Cx26 responded to an increase in P-CO2 at constant extracellular pH by opening and to a decrease in P-CO2 by closing. Furthermore, Cx26 was partially activated at a physiological P-CO2 of around 40 mmHg. Cx26 in isolated patches responded to changes in P-CO2, suggesting direct CO2 sensitivity of the hemichannel to CO2. Heterologous expression of Cx26 in HeLa cells was sufficient to endow them with the capacity to release ATP in a CO2-sensitive manner. We have examined other heterologously expressed connexins for their ability to respond to changes in P-CO2. The closely related beta connexins Cx30 and Cx32 also displayed sensitivity to changes in P-CO2, but with slightly different characteristics from Cx26. The more distant Cx43 exhibited CO2-dependent closing (possibly mediated through intracellular acidification), while Cx36 displayed no CO2 sensitivity. These surprising findings suggest that connexins may play a hitherto unappreciated variety of signalling roles, and that Cx26 and related beta connexins may impart direct sensitivity to CO2 throughout the brain.
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