Journal
JOURNAL OF PHYSIOLOGY-LONDON
Volume 586, Issue 10, Pages 2437-2443Publisher
WILEY-BLACKWELL
DOI: 10.1113/jphysiol.2008.151522
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Funding
- NHLBI NIH HHS [HL 073094, R01 HL073094] Funding Source: Medline
- NIDDK NIH HHS [R21 DK067415, P30 DK079637] Funding Source: Medline
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FRET (Forster resonance energy transfer)-based biosensor molecules are powerful tools to reveal specific molecular interactions in cells. Typically however, they are used in cultured cells that (inevitably) express different genes than their counterparts in intact organisms. In such cells it may be impossible to administer physiological stimuli and measure physiological outputs. Here, through the use of transgenic mice that express a FRET-based myosin light chain kinase (MLCK) biosensor molecule, we report a technique for dynamically observing activation and regulation of MLCK within the smooth muscle cells of intact, functioning small arteries, together with measurement of arterial force production and intracellular [Ca(2+)].
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