Journal
JOURNAL OF PHYSIOLOGICAL SCIENCES
Volume 64, Issue 2, Pages 85-96Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s12576-013-0295-2
Keywords
Colonic motility; Giant migrating contractions; Prostaglandins; EP receptors
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Funding
- Smoking Research Foundation
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The aim of this study was to determine which PGE(2) receptors (EP1-4 receptors) influence colonic motility. Mucosa-free longitudinal smooth muscle strips of the rat middle colon spontaneously induced frequent phasic contractions (giant contractions, GCs) in vitro, and the GCs were almost completely abolished by a cyclooxygenase inhibitor, piroxicam, and by an EP3 receptor antagonist, ONO-AE3-240, but enhanced by tetrodotoxin (TTX). In the presence of piroxicam, exogenous PGE(2), both ONO-AE-248 (EP3 agonist), and ONO-DI-004 (EP1 agonist) induced GC-like contractions, and increased the frequency and amplitude. These effects of EP receptor agonists were insensitive to TTX and omega-conotoxins. In immunohistochemistry, the EP1 and EP3 receptors were expressed in the longitudinal smooth muscle cells. These results suggest that the endogenous PGE(2) spontaneously generates and enhances the frequent phasic contractions directly activating the EP1 and EP3 receptors expressed on longitudinal smooth muscle cells in the rat middle colon.
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