Journal
JOURNAL OF PHYSIOLOGICAL SCIENCES
Volume 63, Issue 2, Pages 147-154Publisher
BMC
DOI: 10.1007/s12576-012-0250-7
Keywords
Dorsal raphe; GABAergic; Serotonin; Electrophysiology
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Funding
- CREST of Japan Science and Technology Agent (JST)
- MEXT
- JSPS
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [21500375, 22590249] Funding Source: KAKEN
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The dorsal raphe nucleus (DRN) is the origin of the central serotonin [5-hydroxytryptamine (5-HT)] system and plays an important role in the regulation of many physiological functions such as sleep/arousal, food intake and mood. In order to understand the regulatory mechanisms of 5-HT system, characterization of the types of neurons is necessary. We performed electrophysiological recordings in acute slices of glutamate decarboxylase 67-green fluorescent protein knock-in mice. We utilized this mouse to identify visually GABAergic cells. Especially, we examined postsynaptic responses mediated by 5-HT receptors between GABAergic and serotonergic cells in the DRN. Various current responses were elicited by 5-HT and 5-HT1A or 5-HT2A/2C receptor agonists in GABAergic cells. These results suggested that multiple 5-HT receptor subtypes overlap on GABAergic cells, and their combination might control 5-HT cells. Understanding the postsynaptic 5-HT feedback mechanisms may help to elucidate the 5-HT neurotransmitter system and develop novel therapeutic approaches.
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