Journal
JOURNAL OF PHYSICS-CONDENSED MATTER
Volume 23, Issue 18, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0953-8984/23/18/184107
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Funding
- German Research Foundation (DFG) [HA 2696/4-3, SPP 1164]
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The mass transfer resistance of a liquid-liquid interface in an aqueous two-phase system composed of poly(ethylene glycol) and dextran is investigated. Different types of proteins and DNA stained with fluorescent dyes serve as probes to study the transport processes close to the interface. A microfluidic device is employed to enable the electrophoretic transport of biomolecules from one phase to another. The results obtained for proteins can be explained solely via the different electrophoretic mobilities and different affinities of the molecules to the two phases, without any indications of a significant mass transfer resistance of the liquid-liquid interface. By contrast, DNA molecules adsorb to the interface and only desorb under an increased electric field strength. The desorption process carries the signature of a thermally activated escape from a metastable state, as reflected in the exponential decay of the fluorescence intensity at the interface as a function of time.
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