Electrochemical Studies on the Effects of Nanometer-Sized Tridecameric Aluminum Polycation on Lactate Dehydrogenase Activity at the Molecular Level

The effects of nanometer-sized tridecameric aluminum polycation (nano-Al-13) on lactate dehydrogenase (LDH). activity have been investigated using differential pulse voltammetry (DPV) at the molecular level. The effects of nano-Al-13 on LDH activity at different temperature and pH values were investigated. The results showed that these factors had quite different influences. The characteristic parameters of Michaelis constant K-m and maximum velocity nu(max) were evaluated by monitoring the DPV reduction current of NAD(+) (beta-nicotinamide adenine dinucleotide) and compared with the other known inhibitors of LDH. The proposed method might be hypothesized to be a simple and convenient approach to evaluate the toxic and biological effects of nanoparticles on the environment.