Journal
JOURNAL OF PHYSICAL CHEMISTRY C
Volume 112, Issue 23, Pages 8629-8633Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp801078m
Keywords
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Funding
- National Research Foundation of Korea [과C6A1905, R15-2004-024-02002-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Detection of single point mutation based on the hybridization of oligonucleotides was performed using unmodified gold nanoparticles. The sequences of oligonucleotides were designed to detect the metastatic efficiency modifier signal-induced proliferation-associated gene 1 (Sipa1). The detection step was monitored using UV-vis absorption spectroscopy, quasielastic light scattering, and zeta potential measurement. We observed that addition of DNAs into the suspension of unmodified gold nanoparticles could substantially aggregate the gold nanoparticles and change the color of solution. By changing the salt concentration in the presence of a phosphate buffer solution, we were able to selectively aggregate gold nanoparticles for the perfectly matched DNA, which enabled a detection of perfectly matched DNA from the single point-mutated one. Our results indicate that a change in the electrostatic interaction is responsible for the selective aggregation of gold nanoparticles upon the addition of DNA. This suggests a novel design principle for a rapid detection of the DNA sequence by controlling the electrostatic interactions between gold nanoparticles.
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