Journal
JOURNAL OF PHYSICAL CHEMISTRY C
Volume 112, Issue 42, Pages 16340-16347Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp804766v
Keywords
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Funding
- FLAD (Luso-American Foundation)
- Fundacao para a Ciencia e a Tecnologia, Portugal [PTDC/QUI/64484/2006, CONC-REEQ/650/2001]
- PhD fellowships [SFRH/BD/18630/2004, SFRH/BD/23604/2005]
- Fundação para a Ciência e a Tecnologia [PTDC/QUI/64484/2006] Funding Source: FCT
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Bionanoconjugates were created with cytochrome c from horse heart (HCc) or yeast (YCc) and citrate-stabilized gold nanoparticles (AuNPs). Evidence for the formation of stable HCc-AuNP and YCc-AuNP bionanoconjugates came from a 5 nm red-shift of the surface plasmon resonance band of the AuNPs, increase of zeta-potential, and direct visualization by atomic force microscopy. Langmuir isotherm fittings of zeta-potential data indicated that higher enthalpy changes are involved in the formation of the HCc-AuNP than in YCc-AuNP. UV-vis and circular dichroism studies of pH-induced aggregation of the bionanoconjugates revealed distinct protonation patterns with an aggregation pH of 8.8 and 6.2 for YCc-AuNP and HCc-AuNP, respectively. No appreciable changes were observed in the secondary structure of HCc in HCc-AuNP. In contrast, YCc in YCc-AuNP presented a decrease in cc-helix content upon AuNP binding and an increase in beta-sheet content upon pH-induced aggregation. Data discussion is based on the distinct binding modes of both proteins to the AuNPs via a covalent bond (Cys 102) for YCc and via electrostatic interaction for HCc.
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