Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 118, Issue 15, Pages 4177-4184Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp500732u
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Funding
- DARPA [QuBE: N66001-10-1-4061]
- Royal Society
- EMF Biological Research Trust
- Grants-in-Aid for Scientific Research [26888004] Funding Source: KAKEN
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Broadband cavity-enhanced absorption spectroscopy (BBCEAS) is shown to be a sensitive method for the detection of magnetic field effects (MFEs) in two flavin-based chemical reactions which are simple models for cryptochrome magnetoreceptors. The advantages of optical cavity-based detection and (pseudo-white-light) super-continuum radiation have been combined to provide full spectral coverage across the whole of the visible spectrum (425 < lambda < 700 nm). This region covers the absorbance spectra of Flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) as well as their photogenerated radicals. To illustrate the power of this technique, BBCEAS has been used to record the spectral dependence of MFEs for photoinduced radical pairs formed in the intermolecular reaction of FMN with lysozyme and the intramolecular photochemistry of FAD. These reactions have been chosen for their photochemical similarities to cryptochrome proteins which have been proposed as key to the magnetic compass sense of many animals including birds. In experiments performed using low protein concentrations (10 mu M) and 1 mm optical path-lengths, absorbance changes as small as 1 x 10(-7) (representing <0.1% MFEs) have been detected with good signal-to-noise offering the prospect of sensitive MFE detection in cryptochrome.
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