Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 118, Issue 14, Pages 3814-3822Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp411387p
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Funding
- Natural Science and Engineering Research Council of Canada (NSERC)
- NSERC
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Polarization-in, polarization-out (PIPO) second harmonic generation (SHG) and third harmonic generation (THG) microscopy was used to study the crystalline organization of beta-carotene molecules within individual aggregates contained in the chromoplasts of orange carrots in vivo. Multimodal PIPO SHG and PIPO THG studies of the aggregates revealed one dominant SHG and THG dipole signifying that beta-carotene molecules are oriented along a single axis. Three-dimensional visualization of the orientation of beta-carotene molecules with respect to the aggregate axis was also performed with both microscopy modalities and revealed organization of the aggregates as ribbon-like structures consisting of twists and folds. Therefore, PIPO SHG and PIPO THG microscopy provides information on the crystalline organization and the orientation of ordered biological structures in vivo where multimodal polarization dependent SHG and THG investigations are particularly advantageous as both noncentrosymmetric and centrosymmetric crystalline organizations can be probed.
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