Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 119, Issue 3, Pages 764-772Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp505127y
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Funding
- ScalaLife (EU)
- CCPBioSim (EPSRC)
- Wellcome Trust
- BBSRC
- Biotechnology and Biological Sciences Research Council [BEP17032, BB/H000267/1, BBS/B/16011, BB/L002558/1, BB/I019855/1, B19456] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J010421/1, EP/M022609/1] Funding Source: researchfish
- BBSRC [BB/I019855/1, BB/L002558/1, BB/H000267/1] Funding Source: UKRI
- EPSRC [EP/M022609/1, EP/J010421/1] Funding Source: UKRI
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Structural studies of membrane proteins have highlighted the likely influence of membrane mimetic environments (i.e., lipid bilayers versus detergent micelles) on the conformation and dynamics of small a-helical membrane proteins. We have used molecular dynamics simulations to compare the conformational dynamics of BM2 (a small alpha-helical protein from the membrane of influenza B) in a model phospholipid bilayer environment with its behavior in proteindetergent complexes with either the zwitterionic detergent dihexanoylphosphatidylcholine (DHPC) or the nonionic detergent dodecylmaltoside (DDM). We find that DDM more closely resembles the lipid bilayer in terms of its interaction with the protein, while the short-tailed DHPC molecule forms nonphysiological interactions with the protein termini. We find that the intrinsic micelle properties of each detergent are conserved upon formation of the proteindetergent complex. This implies that simulations of detergent micelles may be used to help select optimal conditions for experimental studies of membrane proteins.
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