Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 117, Issue 11, Pages 3145-3153Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp401806g
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Funding
- SNF [200021_121579]
- NIH [EY05216]
- Jules Stein Professorship
- Swiss National Science Foundation (SNF) [200021_121579] Funding Source: Swiss National Science Foundation (SNF)
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We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in the Gd(III)-nitroxide pulse double electron-electron resonance (DEER or PELDOR) experiment is reported. A modification of the flip angle of the pump pulse in the Gd(III)-nitroxide DEER experiment is proposed to optimize sensitivity.
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