Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 115, Issue 18, Pages 5493-5498Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp110924w
Keywords
-
Categories
Funding
- NSF [0806266]
- ARO DOD [W911NF-091-04880]
- SMART ID-IRG
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0806266] Funding Source: National Science Foundation
Ask authors/readers for more resources
Many enzymatic reactions in biochemistry are far more complex than the celebrated Michaelis-Menten scheme, but the observed turnover rate often obeys the hyperbolic dependence on the substrate concentration, a relation established almost a century ago for the simple Michaelis-Menten mechanism. To resolve the longstanding puzzle, we apply the flux balance method to predict the functional form of the substrate dependence in the mean turnover time of complex enzymatic reactions and identify detailed balance (i.e., the lack of unbalanced conformtional current) as a sufficient condition for the Michaelis Menten equation to describe the substrate concentration dependence of the turnover rate in an enzymatic network. This prediction can be verified in single-molecule event-averaged measurements using the recently proposed signatures of detailed balance violations. The finding helps analyze recent single-molecule studies of enzymatic networks and can be applied to other external variables, such as force-dependence and voltage-dependence.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available