Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 114, Issue 25, Pages 8574-8580Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp1029536
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Funding
- The University of Akron
- Coleman endowment
- The National Science Foundation [CHE-9977144]
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A series of squaraine dyes are found to be in H- or J-aggregates and are almost nonfluorescent in aqueous solution. Upon addition of bovine serum albumin (BSA), however, the fluorescence intensity (at lambda(em) approximate to 690 nm) increases by a factor of up to 200. Transformation of the dye molecules from the aggregates to the monomeric species appears to be responsible for the large fluorescence turn-on. While both H- and J-aggregates contribute to the observed fluorescence turn-on, the former appears to play a more important role. Electrophoresis imaging shows that these probes are good BSA indicators.
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