Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 113, Issue 19, Pages 6854-6860Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp901542b
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Funding
- National Institutes of Health [ROI GM67891]
- National Science Foundation [CHE-0303715]
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The kinetics of biomolecular conformational transitions can be studied by two-dimensional (213) magnetic resonance and optical spectroscopic methods. Here we apply polarization-modulated Fourier imaging correlation spectroscopy (PM-FICS) to demonstrate a new approach to 2D optical spectroscopy. PM-FICS enables measurements of conformational fluctuations of fluorescently labeled macromolecules on a broad range of time scales (10(-3)-10(2) S). We examine the optical switching pathways of DsRed, a tetrameric complex of fluorescent protein subunits. An analysis of PM-FICS coordinate trajectories, in terms of 2D spectra and joint probability distributions, provides detailed information about the transition pathways between distinct dipole-coupled DsRed conformations.
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