Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 113, Issue 18, Pages 6520-6527Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp810397u
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Funding
- Danish Natural Science Research Council
- Swiss National Science Foundation
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We demonstrate the extension of the principle of difference Fourier transform infrared (FTIR) spectroscopy to difference 2D-IR spectroscopy. To this end, we measure difference 2D-IR spectra of the protein bacteriorhodopsin in its early J- and K-intermediates. By comparing with the static 2D-IR spectrum of the protonated Schiff base of all-trans retinal, we demonstrate that the 2D-IR spectrum of the all-trans retinal chromophore in bacteriorhodopsin can be measured with the background from the remainder of the protein completely suppressed. We discuss several models to interpret the detailed line shape of the difference 2D-IR spectrum.
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