Journal
JOURNAL OF PHYSICAL CHEMISTRY A
Volume 113, Issue 16, Pages 4639-4646Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp811395r
Keywords
-
Funding
- Department of Energy [DE-FG02-02ER45995]
- University of Florida Alumni Fellowship
- Research Corporation Award [CC5479]
- Petroleum Research Fund
- American Chemical Society
- Towsley Foundation
- Large Allocations Resource Committee [TG-MCA05S010, TGCHE050058T]
- U.S. Department of Energy (DOE) [DE-FG02-02ER45995] Funding Source: U.S. Department of Energy (DOE)
Ask authors/readers for more resources
FRET experiments enable studies of the chemical and physical properties of individual molecules, which has long been a dream of chemists. However, these modem experimental techniques are still limited by the lack of information about the dynamic behavior of the fluorescent labels as well as by the use of dipole-dipole approximation even at short donor-to-acceptor distances. Our results help to suggest that these assumptions need to be carefully considered when designing experiments. We show that at short donor-acceptor separation, dipole-dipole approximation breaks down and Forster theory fails and cannot be used to obtain correct distances. We also explicitly demonstrate that dyes' linkers allow for a lot of flexibility in the fluorescent label orientation and position resulting in distances much shorter than assumed earlier.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available