Journal
JOURNAL OF PHYSICAL CHEMISTRY A
Volume 112, Issue 37, Pages 8654-8658Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp8026226
Keywords
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Funding
- Department of Chemistry and Biochemistry, University of Lethbridge
- National Merit Finalist Presidential Scholarship from the University of Southern California
- National Science Foundation [0513701]
- Direct For Computer & Info Scie & Enginr
- Div Of Information & Intelligent Systems [0513701] Funding Source: National Science Foundation
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In the application of the quasi-steady-state approximation, it is generally assumed that there is an initial transient during which the substrate concentration remains approximately constant while the complex concentration builds up. In this paper, we address the assumption that the substrate concentration does not change significantly during this initial transient and name it the reactant stationary approximation. For the single enzyme, single substrate reaction, the reactant stationary approximation is generally considered a sufficient condition to apply the quasi-steady-state approximation. Studying the dynamic behavior of this reaction with endogenous substrate, we show that the quasi-steady-state approximation and reactant stationary approximation are two separate approximations. We discuss the consequence of this result for the determination of reaction parameters in enzyme catalyzed reactions.
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