Journal
JOURNAL OF PHYCOLOGY
Volume 49, Issue 6, Pages 1181-1191Publisher
WILEY-BLACKWELL
DOI: 10.1111/jpy.12129
Keywords
acclimation; hypersalinity stress; polyamine; protein disulfide isomerase; Ulva lactuca
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Funding
- National Science Council, Executive Yuan, Taiwan [NSC 96-2311-B-110-002-MY3, NSC 99-2311-B-110-001-MY3, NSC 98-3114-B-110-001-, NSC 99-3113-P-110-001-, NSC 100-3113-P-110-001-]
- Asia-Pacific Ocean Research Center of National Sun Yat-sen University
- Ministry of Education, Executive Yuan, Taiwan, under the Grant-Aim for the Top University Plan
- Academia Sinica, Taipei, Taiwan
- National Sun Yat-sen University, Kaohsiung, Taiwan
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Full-length protein disulfide isomerase (UfPDI) cDNA was cloned from the intertidal macroalga Ulva lactuca Linnaeus. Modulation of UfPDI expression by stresses and polyamines (PA) was studied. UfPDI transcription and enzyme activity were increased by hypersalinity (90) or high light illumination (1,200molphotonsm(-2)s(-1)), decreased by the addition of 100M CuSO4. An exposure to a salinity of 90 decreased PA contents. Treating with PA biosynthetic inhibitors, D-arginine (D-Arg) or -methyl ornithine (-MO), led to a further decrease and also inhibited UfPDI expression and recovery of the growth rate. These results suggest that PAs are required to activate UfPDI expression with hypersalinity, even PA contents are decreased at a salinity of 90. The induction of UfPDI expression by hypersalinity of 90 and tolerance to hypersalinity could be enhanced if internal PA contents rise. Sung etal. (2011b) showed that PA contents could be increased by pretreating with putrescine (Put, 1mM), spermidine (Spd, 1mM), or spermine (Spm, 1mM) at a salinity of 30. Therefore, PA pretreatment effect on UfPDI expression was examined. Pretreatment with Spd and Spm, but not with Put, enhanced UfPDI expression after transferred to a salinity of 90 and restored the growth rate. In conclusion, induction of UfPDI expression by Spd or Spm before exposure to hypersaline conditions and continuous up-regulation after hypersalinity exposure are required for the acquisition of hypersalinity tolerance in the intertidal green macroalga U.lactuca.
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