Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 99, Issue 12, Pages 5203-5215Publisher
SPRINGER
DOI: 10.1007/s00253-015-6440-5
Keywords
Haematococcus pluvialis; Photoautotrophic induction; Astaxanthin; High temperature; Haber-Weiss reaction
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Funding
- Korea Institute of Energy Technology Evaluation and Planning
- Ministry of Trade, Industry and Energy of Korea as a part of the Project of Process demonstration for bioconversion of CO2 to high-valued biomaterials using microalgae in Energy Efficiency & Resources Technology RD project [20122010200010-11-2-100]
- National Research Foundation of Korea (NRF) [NRF-2013R1A2A1A01015644/2010-0027955]
- Korea CCS R&D Center of the NRF - Ministry of Science, ICT, and Future Planning of Korea [2014M1A8A1049278]
- NLRL (National Leading Research Lab.) Project [2012R1A2A1A01008085]
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High temperatures (30-36 A degrees C) inhibited astaxanthin accumulation in Haematococcus pluvialis under photoautotrophic conditions. The depression of carotenogenesis was primarily attributed to excess intracellular less reactive oxygen species (LROS; O-2 (-) and H2O2) levels generated under high temperature conditions. Here, we show that the heat stress-driven inefficient astaxanthin production was improved by accelerating the iron-catalyzed Haber-Weiss reaction to convert LROS into more reactive oxygen species (MROS; O-2 and OH center dot), thereby facilitating lipid peroxidation. As a result, during 18 days of photoautotrophic induction, the astaxanthin concentration of cells cultured in high temperatures in the presence of iron (450 mu M) was dramatically increased by 75 % (30 A degrees C) and 133 % (36 A degrees C) compared to that of cells exposed to heat stress alone. The heat stress-driven Haber-Weiss reaction will be useful for economically producing astaxanthin by reducing energy cost and enhancing photoautotrophic astaxanthin production, particularly outdoors utilizing natural solar radiation including heat and light for photo-induction of H. pluvialis.
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